phospho stat3  (Bioss)

Journal: Bioactive Materials

Article Title: One-step strategy for fabricating icariin-encapsulated biomimetic Scaffold: Orchestrating immune, angiogenic, and osteogenic cascade for enhanced bone regeneration

doi: 10.1016/j.bioactmat.2025.06.001

Figure Lengend Snippet: (A) One-step strategy prepared CHP@IC scaffold containing PLGA@IC microspheres. (B) CHP@IC activates immune-mediated vascular-bone regeneration cascade via STAT3 signaling pathway.

Article Snippet: Then, the membrane was blocked with 5 % skimmed milk and incubated with iNOs (NB300-605, Novus, USA), ARG1 (16001-1-AP, Proteintech, UK), STAT3 (ET1607-38, HUABIO, China), phospho-STAT3 antibodies (S727) (ET1607-39, HUABIO, China) and GAPDH (ab8245, Abcam, UK) overnight.

Techniques:

Journal: Bioactive Materials

Article Title: One-step strategy for fabricating icariin-encapsulated biomimetic Scaffold: Orchestrating immune, angiogenic, and osteogenic cascade for enhanced bone regeneration

doi: 10.1016/j.bioactmat.2025.06.001

Figure Lengend Snippet: CHP@IC activates the STAT3 signaling pathway. (A) Schematic representation of CHP@IC activation of the STAT3 signaling pathway. (B–C) The WB results and the relative protein expression of STAT3, p-STAT3 and p-STAT3/STAT3 treated with CHP@IC scaffold. (D) The WB results of STAT3, p-STAT3 and p-STAT3/STAT3 treated with/without NSC 74859. (E) The WB results of iNOs and ARG1 treated with/without NSC 74859. (F)The quantitative analysis of protein expression with/without NSC 74859. (G–H) The RT-qPCR results of macrophages treated with/without NSC 74859.

Article Snippet: Then, the membrane was blocked with 5 % skimmed milk and incubated with iNOs (NB300-605, Novus, USA), ARG1 (16001-1-AP, Proteintech, UK), STAT3 (ET1607-38, HUABIO, China), phospho-STAT3 antibodies (S727) (ET1607-39, HUABIO, China) and GAPDH (ab8245, Abcam, UK) overnight.

Techniques: Activation Assay, Expressing, Quantitative RT-PCR